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Submitted: 23 Jul 2025
Revision: 16 Oct 2025
Accepted: 31 Oct 2025
ePublished: 09 Nov 2025
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J Nephropharmacol. Inpress.
doi: 10.34172/npj.2025.12808
  Abstract View: 22

Original

Association between anti-erythropoietin antibody levels and treatment response in hemodialysis patients with end-stage renal disease: A cross-sectional study from Iraq

Samer Imad Mohammed 1* ORCID logo, Mohammed Yawuz Jamal 1 ORCID logo, Kawthar Faris Nassir 2 ORCID logo, Zahraa Duraid Abdulazeez 3 ORCID logo

1 Department of Clinical Pharmacy, College of Pharmacy, University of Baghdad, Baghdad, Iraq
2 Department of Clinical Pharmacy, Therapeutic Drug Monitoring Center-Baghdad Teaching Hospital, Medical City, Baghdad, Iraq
3 Department of Pharmaceutical Chemistry, College of Pharmacy, University of Baghdad, Baghdad, Iraq
*Corresponding Author: Samer I. Mohammed, Email: samer.jameel@copharm.uobaghdad.edu.iq

Abstract

Introduction: The therapeutic efficacy of erythropoietin (EPO) may be diminished by the formation of anti-erythropoietin antibodies (AEAs), potentially resulting in therapy failure.

Objectives: This retrospective study included 84 hemodialysis patients with end-stage renal disease (ESRD) treated with recombinant EPO and aimed to evaluate the association between serum AEA and treatment response.

Patients and Methods: This cross-sectional study included eighty-four Iraqi patients with ESRD who had been on recombinant human EPO for more than 6 months and had hemoglobin levels below 11 g/dL. Serum AEA levels were measured in all patients to evaluate their association with treatment response and to investigate correlations with demographic, clinical, and laboratory data.

Results: The study found that 64.3% of patients displayed increased AEA levels (>20 ng/mL). A poorer hemoglobin response after two months (ΔHb) was associated with increased AEA levels (β = -15.21, P=0.002). Additionally, a higher erythropoietin resistance index (ERI) emerged as a significant positive predictor (β = 1.50, P=0.018), while lower serum albumin demonstrated a protective effect (β = -22.50, P=0.048).

Conclusion: This study indicates a significant prevalence of AEA in patients with ESRD undergoing EPO therapy. Regression analysis revealed that a poorer hemoglobin response (ΔHb) and a higher ERI were independent predictors of elevated AEA titers, while serum albumin was a protective factor.


Implication for health policy/practice/research/medical education:

The significant prevalence of anti-erythropoietin antibodies (AEAs) among Iraqi hemodialysis patients necessitates a policy change to implement targeted screening, focusing on individuals exhibiting a high erythropoietin resistance index (ERI) or inadequate hemoglobin response (ΔHb), which are the primary predictors identified in the study. Monitoring these metrics and optimizing nutritional status, such as serum albumin, is clinically essential. Research should focus on investigating the underlying drivers of AEA in this population. Medical education should incorporate AEA pathophysiology and the analysis of ERI and ΔHb to enhance the identification and management of antibody-mediated treatment resistance.

Please cite this paper as: Mohammed SI, Jamal MY, Nassir KF, Abdulazeez ZD. Association between anti-erythropoietin antibody levels and treatment response in hemodialysis patients with end-stage renal disease: a cross-sectional study from Iraq. J Nephropharmacol. 2025;14(x):e12808. DOI: 10.34172/npj.2025.12808.

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