Society of Diabetic Nephropathy Prevention Journal of Nephropharmacology 2345-4202 8 1 2019 01 01 The protective effects of vasopressin on experimental testicular torsion-detorsion in male rats e10 e10 10.15171/npj.2019.10 EN Amin Hasanvand Fariba Ahmadizar Sadegh Ebrahimi-Ghalaei Fatemeh Beyranvand Reza Mohammadrezaei Khorramabadi Peyman Amanolahi Baharvand Abolfazl Abbaszadeh Journal Article 10.15171/npj.2019.10 2018 10 12 2018 12 10 Introduction: Vasopressin is a potent vasoconstrictor. Vasopressin can reduce damages caused by ischemia-reperfusion. Objectives: The aim of this study was to evaluate the reducing effect of vasopressin on the damage caused by testicular torsion-detorsion in male rats. Materials and Methods: Fifty male rats were used in this study. The rats were divided randomly into five groups (10 per group) as follows; 1) control group (without surgery), 2) sham group (surgery without torsion-detorsion), 3) torsion-detorsion surgery without any treatment, 4) torsion-detorsion surgery with vasopressin treatment (0.1 μg/kg), 5) torsion-detorsion surgery with vasopressin treatment (0.2 μg/kg). After that, the rats were killed and subsequently the biochemical parameters were measured by commercial kits. Histological changes were assessed by H&E staining. Results: Using Kruskal Wallis test, we observed that the changes in the tissues of seminiferous tubes, testosterone levels, glutathione (GSH) and thioredoxin enzymes in the studied groups (control, sham, torsion-detorsion, torsion-detorsion with 0.1 μg/kg, torsion-detorsion with 0.2 μg/kg) were statistically significant. Conclusion: Intracellular mechanisms of vasopressin involved in the regulation of protective mechanism and can reduce damages caused by ischemia-reperfusion. Vasopressin improves the process of changes in the testicular seminiferous tubes of rats with torsion-detorsion. Moreover, it also increases serum levels of testosterone, GSH and thioredoxin enzymes compared to the torsiondetorsion group