Abstract
Introduction: Vasopressin is a potent vasoconstrictor. Vasopressin can reduce damages caused by
ischemia-reperfusion.
Objectives: The aim of this study was to evaluate the reducing effect of vasopressin on the damage
caused by testicular torsion-detorsion in male rats.
Materials and Methods: Fifty male rats were used in this study. The rats were divided randomly into
five groups (10 per group) as follows; 1) control group (without surgery), 2) sham group (surgery
without torsion-detorsion), 3) torsion-detorsion surgery without any treatment, 4) torsion-detorsion
surgery with vasopressin treatment (0.1 μg/kg), 5) torsion-detorsion surgery with vasopressin
treatment (0.2 μg/kg). After that, the rats were killed and subsequently the biochemical parameters
were measured by commercial kits. Histological changes were assessed by H&E staining.
Results: Using Kruskal Wallis test, we observed that the changes in the tissues of seminiferous tubes,
testosterone levels, glutathione (GSH) and thioredoxin enzymes in the studied groups (control,
sham, torsion-detorsion, torsion-detorsion with 0.1 μg/kg, torsion-detorsion with 0.2 μg/kg) were
statistically significant.
Conclusion: Intracellular mechanisms of vasopressin involved in the regulation of protective
mechanism and can reduce damages caused by ischemia-reperfusion. Vasopressin improves the
process of changes in the testicular seminiferous tubes of rats with torsion-detorsion. Moreover, it
also increases serum levels of testosterone, GSH and thioredoxin enzymes compared to the torsiondetorsion group