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Submitted: 12 Oct 2018
Accepted: 10 Dec 2018
First published online: 06 Jan 2018
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J Nephropharmacol. 2019;8(1): e10.
doi: 10.15171/npj.2019.10
  Abstract View: 610
  PDF Download: 361

Original

The protective effects of vasopressin on experimental testicular torsion-detorsion in male rats

Amin Hasanvand 1, Fariba Ahmadizar 2, Sadegh Ebrahimi-Ghalaei 3, Fatemeh Beyranvand 4, Reza Mohammadrezaei Khorramabadi 3, Peyman Amanolahi Baharvand 4, Abolfazl Abbaszadeh 5 *

1 Nutritional Health Research Center, Department of Pharmacology and toxicology, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran
2 Department of Epidemiology, Erasmus University Medical Center, Rotterdam, the Netherlands
3 Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
4 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
5 Department of Surgery, Lorestan University of Medical Sciences, Khorramabad, Iran
*Corresponding author: Abolfazl Abbaszadeh, Ph.D; Email: Email: Abbaszadeh.a@lums.ac.ir

Abstract

Introduction: Vasopressin is a potent vasoconstrictor. Vasopressin can reduce damages caused by ischemia-reperfusion.

Objectives: The aim of this study was to evaluate the reducing effect of vasopressin on the damage caused by testicular torsion-detorsion in male rats.

Materials and Methods: Fifty male rats were used in this study. The rats were divided randomly into five groups (10 per group) as follows; 1) control group (without surgery), 2) sham group (surgery without torsion-detorsion), 3) torsion-detorsion surgery without any treatment, 4) torsion-detorsion surgery with vasopressin treatment (0.1 μg/kg), 5) torsion-detorsion surgery with vasopressin treatment (0.2 μg/kg). After that, the rats were killed and subsequently the biochemical parameters were measured by commercial kits. Histological changes were assessed by H&E staining.

Results: Using Kruskal Wallis test, we observed that the changes in the tissues of seminiferous tubes, testosterone levels, glutathione (GSH) and thioredoxin enzymes in the studied groups (control, sham, torsion-detorsion, torsion-detorsion with 0.1 μg/kg, torsion-detorsion with 0.2 μg/kg) were statistically significant.

Conclusion: Intracellular mechanisms of vasopressin involved in the regulation of protective mechanism and can reduce damages caused by ischemia-reperfusion. Vasopressin improves the process of changes in the testicular seminiferous tubes of rats with torsion-detorsion. Moreover, it also increases serum levels of testosterone, GSH and thioredoxin enzymes compared to the torsiondetorsion group

Keywords: Ischemia-reperfusion, Testicular rotation, Testis, Glutathione, Reactive oxygen species, Ischemia-reperfusion

Implication for health policy/practice/research/medical education:

Testicular torsion is an urgent condition in pediatric urology. Testicular torsion refers to testicular rotation around its vascular axis (spermatic cord), which results in obstruction and stoppage of blood flow and eventually the loss of living tissue (infarction). Ischemic condition causes damage to the testes due to the blood flow disruption. Furthermore, reperfusion of blood flow after ischemia (detorsion) can exacerbate the damage to the testis. Vasopressin as an antidiuretic hormone (ADH), is a pituitary hormone found in most mammals including humans. It is mainly made in supra-optic nuclei. Vasopressin is a polypeptide hormone with nine amino acids and is similar to the oxytocin hormone. In an experimental study, we found that vasopressin possesses intracellular mechanisms similar to the protective preconditioning mechanism which can reduce damages caused by ischemia-reperfusion.

Please cite this paper as: Hasanvand A, Ahmadizar F, Ebrahimi-Ghalaei S, Beyranvand F, Mohammadrezaei Khorramabadi R, Amanolahi Baharvand P, et al. The protective effects of vasopressin on experimental testicular torsion-detorsion in male rats. J Nephropharmacol. 2019;8(1):e10. DOI: 10.15171/npj.2019.10

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